Novel mouse models for use in IL-17A and Th17 research

Th17 cells have emerged as a proinflamatory cell type with strong links to autoimmunity and immunopathology. The aims of this thesis are two-fold; Firstly, generation of a novel mouse model that allows in vivo and/or ex vivo observation and manipulation of Th17 cells. Secondly, to generate a mouse model capable of conditionally overexpressing the hallmark Th17 cytokine, IL-17A. Given the expertise and experience in our lab with respect to conditional gene targeting, Cre-LoxP-mediated approaches were chosen and utilized to achieve this goal in both mouse models. The resulting strains and the knowledge generated from their useage are discussed in this work. Furthermore, the recently generated IL-6Rα conditional allele allows for ablation of IL-6 signaling in a cell type-specific manner. We wanted to analyze the role of IL-6 signaling with respect to EAE pathogenesis and development of pathogenic Th17 cells, and the results generated are published in this work.